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  • Title: A highly selective fluorescent chemosensor for zinc ion and imaging application in living cells.
    Author: Saha UC, Chattopadhyay B, Dhara K, Mandal SK, Sarkar S, Khuda-Bukhsh AR, Mukherjee M, Helliwell M, Chattopadhyay P.
    Journal: Inorg Chem; 2011 Feb 21; 50(4):1213-9. PubMed ID: 21247079.
    Abstract:
    A new 2,6-bis(5,6-dihydrobenzo[4,5]imidazo[1,2-c]quinazolin-6-yl)-4-methylphenol (1) serves as a highly selective and sensitive fluorescent probe for Zn(2+) in a HEPES buffer (50 mM, DMSO:water = 1:9 (v/v), pH = 7.2) at 25 °C. The increase in fluorescence in the presence of Zn(2+) is accounted for by the formation of dinuclear Zn(2+) complex [Zn(2)(C(35)H(25)N(6)O)(OH)(NO(3))(2)(H(2)O)] (2), characterized by X-ray crystallography. The fluorescence quantum yield of the chemosensor 1 is only 0.019, and it increases more than 12-fold (0.237) in the presence of 2 equiv of the zinc ion. Interestingly, the introduction of other metal ions causes the fluorescence intensity to be either unchanged or weakened. By incubation of cultured living cells (A375 and HT-29) with the chemosensor 1, intracellular Zn(2+) concentrations could be monitored through selective fluorescence chemosensing.
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