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  • Title: Single-molecule four-color FRET visualizes energy-transfer paths on DNA origami.
    Author: Stein IH, Steinhauer C, Tinnefeld P.
    Journal: J Am Chem Soc; 2011 Mar 30; 133(12):4193-5. PubMed ID: 21250689.
    Abstract:
    Fluorescence resonance energy transfer (FRET) represents a mechanism to transport light energy at the nanoscale, as exemplified by nature's light-harvesting complexes. Here we used DNA origami to arrange fluorophores that transport excited-state energy from an input dye to an output dye. We demonstrate that energy-transfer paths can be controlled on the single-molecule level by the presence of a "jumper" dye that directs the excited-state energy either to a red or to an IR output dye. We used single-molecule four-color FRET with alternating laser excitation to sort subpopulations and to visualize the control of energy transfer.
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