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Title: [Production of pyoverdin by Pseudomonas fluorescens in human blood at 4 degrees C: association with proteins and the cytotoxic effect of the pigment]. Author: Pájaro MC, Albesa I. Journal: Rev Argent Microbiol; 1990; 22(1):24-30. PubMed ID: 2125740. Abstract: Pyoverdin was purified from Pseudomonas fluorescens cultured in a synthetic medium. Cytotoxic effect on human leukocytes was assayed. Death and lysis was observed depending on concentration and time. Sub-lytic dose decreased leukocyte phagocytosis. The pigment was produced by P. fluorescens in blood stored at 4 degrees C. Saline precipitation of plasma showed that globulin and albumin fraction retained 46% and 37% of pyoverdin, respectively. By means of dialysis it was possible to determine that albumin retained more pigment than the other fractions either bound to the protein or aggregated.[Abstract] [Full Text] [Related] [New Search]