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  • Title: Heterotrophic plate counts and the isolation of bacteria from mineral waters on selective and enrichment media.
    Author: Manaia CM, Nunes OC, Morais PV, da Costa MS.
    Journal: J Appl Bacteriol; 1990 Dec; 69(6):871-6. PubMed ID: 2126789.
    Abstract:
    The heterotrophic plate counts of 15 brands of bottled non-carbonated mineral waters were examined and found to be generally high and variable. Four selective or enrichment media for the enumeration of coliforms (m-Endo LES and m-lauryl sulphate agar) and Pseudomonas aeruginosa (cetrimide-nalidixic acid agar and malachite green broth) were used to isolate several species of Gram-negative bacteria. Strains identified as CDC gr IVc-2 and Comamonas (Ps.) acidovorans were the two most commonly isolated. Considerable variation in populations was seen between the brands, as well as between two batches of the same mineral water.
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