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  • Title: Production of the seed germination stimulant karrikinolide from combustion of simple carbohydrates.
    Author: Flematti GR, Scaffidi A, Dixon KW, Smith SM, Ghisalberti EL.
    Journal: J Agric Food Chem; 2011 Feb 23; 59(4):1195-8. PubMed ID: 21280622.
    Abstract:
    The naturally occurring seed germination stimulant karrikinolide is formed from the combustion of plant material including cellulose. It has previously been reported that combustion of simple carbohydrates such as d-glucose does not produce extracts containing karrikinolide. Moreover, it was reported that extracts with germination-promoting ability could be obtained only by combustion of simple carbohydrates in the presence of amino acids such as l-glycine. By employing a (13)C-labeled karrikinolide to physically quantify natural karrikinolide, we now show that it is produced from combustion of simple carbohydrates in similar amounts regardless of whether l-glycine is present or not. The addition of l-glycine appears to be beneficial in reducing the inhibitory effect of smoke extracts and provides a greater concentration range for effective germination-promoting activity.
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