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  • Title: [Synthesis of new fluorogenic substrates derivated of 7-amino-4-trifluoromethylcoumarin. Detection of gram-negative bacteria, Streptococci group A and Enterococci].
    Author: Agban A, Ounanian M, Luu-Duc C, Monget D.
    Journal: Ann Pharm Fr; 1990; 48(6):326-34. PubMed ID: 2131765.
    Abstract:
    The synthesis of substrates having 7-amino 4-trifluoromethyl coumarin as fluorogenic reagent is described. The first group is performed from L-alanine derivative in order to investigate L-alanine aminopeptidase activity for the detection of Gram-negative bacteria. The second group is synthesized from the L-pyrrolidon carboxylic acid. It allows us to detect the PYRase activity of group A Streptococci and Enterococci. These substrates are evaluated with the N-protected form and the amino free one.
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