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  • Title: [Relationship between the reaching of equilibrium and the accuracy of the affinity determination for antibody-antigen reaction].
    Author: Bobrovnik SA, Demchenko MA, Komisarenko SV.
    Journal: Ukr Biokhim Zh (1999); 2010; 82(3):68-74. PubMed ID: 21328880.
    Abstract:
    Theoretical investigation of the relationship between the reaching of equilibrium and the accuracy of the affinity determination for antibody-antigen reaction were carried out. It was shown that evaluation of the reagent concentrations before reaching the dynamic equilibrium leads to a decrease of estimation accuracy for the affinity constant of this reaction. We have established that for most of typical antibodies the dynamic equilibrium of their reaction with corresponding antigens comes 2-4 hours after the beginning.
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