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  • Title: FRET-based biosensor for oleic acid in nanomolar range with quantum dots as an energy donor.
    Author: Dezhurov SV, Volkova IY, Wakstein MS.
    Journal: Bioconjug Chem; 2011 Mar 16; 22(3):338-45. PubMed ID: 21332206.
    Abstract:
    A self-assembling sensor for oleic acid has been developed. The sensor consists of a self-assembling fluorescent dye labeled BSA and quantum dots CdSe/ZnS capped with 3-mercaptopropionic acid. The detection limit of the new sensor is 10-1000 nM. The influence of the quantum dot size on the FRET efficiency in the course of the interaction of the sensor system with the analyte has been studied. The pH dependence, aggregation stability. and electrophoretic properties of the sensor have been examined. The data suggest a new approach for the development of nanoscale FRET-based sensors operating effectively due to unique fluorescent properties of quantum dots as well as due to selective protein-ligand interactions.
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