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  • Title: Colorimetric assay of HIV-1 proteinase suitable for high-capacity screening.
    Author: Billich A, Winkler G.
    Journal: Pept Res; 1990; 3(6):274-6. PubMed ID: 2134071.
    Abstract:
    The cleavage of substrate peptides by HIV-1 proteinase can be monitored by reaction of exposed amino groups with 2,4,6-trinitrobenzenesulfonic acid to yield yellow-colored products. This method provides a high-capacity assay of the enzyme which can be performed on microtiter plates.
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