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  • Title: Characterization of a novel temperate phage originating from a cereulide-producing Bacillus cereus strain.
    Author: Smeesters PR, Drèze PA, Bousbata S, Parikka KJ, Timmery S, Hu X, Perez-Morga D, Deghorain M, Toussaint A, Mahillon J, Van Melderen L.
    Journal: Res Microbiol; 2011 May; 162(4):446-59. PubMed ID: 21349326.
    Abstract:
    A novel temperate bacteriophage was isolated from a Bacillus cereus cereulide-producing strain and named vB_BceS-IEBH. vB_BceS-IEBH belongs to the Siphoviridae family. The complete genome sequence (53 kb) was determined and annotated. Eighty-seven ORFs were detected and for 28, a putative function was assigned using the ACLAME database. vB_BceS-IEBH replicates as a plasmid in the prophage state. Accordingly, a 9-kb plasmid-like region composed of 13 ORFs was identified. A fragment of around 2000 bp comprising an ORF encoding a putative plasmid replication protein was shown to be self-replicating in Bacillus thuringiensis. Mass spectrometry analysis of the purified vB_BceS-IEBH particle identified 8 structural proteins and enabled assignment of a supplementary ORF as being part of the morphogenesis module. Genome analysis further illustrates the diversity of mobile genetic elements and their plasticity within the B. cereus group.
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