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  • Title: Effect of alkaline pre-treatment on enzyme synergy for efficient hemicellulose hydrolysis in sugarcane bagasse.
    Author: Beukes N, Pletschke BI.
    Journal: Bioresour Technol; 2011 Apr; 102(8):5207-13. PubMed ID: 21353533.
    Abstract:
    This aim of this study was to investigate the effect of ammonium hydroxide (NH(4)OH) and sodium hydroxide (NaOH) pre-treatment on the digestibility of sugarcane bagasse (SCB) by hemicellulase action. It was found that pre-treatment of SCB with NH(4)OH removed a larger percentage of the SCB lignin and effectively increased SCB digestibility 13.13 fold. The greatest amount of reducing sugar (1194.88 μmol/min) and largest degree of synergy (2.85) was obtained using a combination of two enzymes (25% ManA and 75% XynA) with NH(4)OH pre-treated SCB. In this study, NH(4)OH therefore appeared to be a more effective pre-treatment step for subsequent hydrolysis by hemicellulases.
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