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  • Title: Expansion of intracellular IFN-γ positive lymphocytes during Mycoplasma agalactiae infection in sheep.
    Author: La Manna MP, Agnone A, Villari S, Puleio R, Vitale M, Nicholas R, Sireci G, Dieli F, Loria GR.
    Journal: Res Vet Sci; 2011 Dec; 91(3):e64-7. PubMed ID: 21354587.
    Abstract:
    A method to assess the expansion of antigen-specific intracellular IFN-γ positive T cell subsets during the infection will be helpful for a better understanding of mycoplasmal infections physiopathology in the sheep. We analysed the percentage of antigen-specific lymphocytes positive for intracellular IFN-γ during the infection of sheep with Mycoplasma agalactiae by culturing peripheral blood mononuclear cells of infected or uninfected animals with irradiated M. agalactiae. The expansion of antigen-specific IFN-γ positive lymphocytes in infected sheep was initially sustained by CD4(+) T cells at day 15 after infection, when antigen specific IgG start to be detectable, followed by CD8/IFN-γ double positive cells. γδ T-cells were not expanded at any time point analysed. IFNγ(+) T cells disappear 60 days after infection, suggesting that antigen specific IFNγ(+) T cells, mainly detected in the early phase of the disease, could be useful to understand the role of cell-mediated immunity during M. agalactiae infection.
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