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  • Title: Purification of tumor mitochondrial malic enzyme by specific ligand affinity chromatography.
    Author: Teller JK, Fahien LA.
    Journal: Protein Expr Purif; 1990 Nov; 1(2):151-4. PubMed ID: 2136236.
    Abstract:
    A two-step chromatographic procedure, based on a specific ligand-binding approach, for the purification of tumor NAD(P)(+)-dependent malic enzyme is described. The enzyme was purified to near homogeneity by extraction from mitochondria, negative cellulose phosphate chromatography, ammonium sulfate precipitation, and application of specific elution from a malate-agarose column. The rationale for the use of the affinity column is also described.
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