These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Evaluation of deuterium labeled and unlabeled bis-methyl glutathione combined with nanoliquid chromatography-mass spectrometry to screen and characterize reactive drug metabolites.
    Author: Defoy D, Dansette PM, Neugebauer W, Wagner JR, Klarskov K.
    Journal: Chem Res Toxicol; 2011 Mar 21; 24(3):412-7. PubMed ID: 21375241.
    Abstract:
    We present a reactive metabolite detection assay based on the use of deuterium labeled/unlabeled bis-methyl glutathione (GSH) esters (GSH(CH(3)/CD(3))(2)) and nanoliquid chromatography coupled online with electrospray ionization tandem mass spectrometry (nLC-ESI-MS/MS). Compared with glutathione, neutralization of the carboxylic acid groups by esterification introduced a mass difference of 6, which facilitated the identification of trapped metabolites and improved the intensity of the mass spectrometry signal in positive ionization mode. The peptides allowed for the trapping of soft electrophilic reactive metabolites generated in vitro by incubation with acetaminophen, carbamazepine (CBZ), NADPH, and microsomes.
    [Abstract] [Full Text] [Related] [New Search]