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  • Title: A simple and rapid procedure for purification of haptoglobin from human plasma fraction IV.
    Author: Sun L, Huang Y, Zhang Y, Meng Q, Luo J, Fan B, Ma G, Su Z.
    Journal: Artif Cells Blood Substit Immobil Biotechnol; 2011 Apr; 39(2):79-86. PubMed ID: 21375374.
    Abstract:
    Human plasma fraction IV is an intermediate precipitate during the production of human serum albumin using cold ethanol method. Haptoglobin locates in this fraction can be purified for various applications. A new process integration of polyethylene glycol (PEG) precipitation and ion-exchange chromatography (IEC) was developed for purification of haptoglobin, which could effectively purify the haptoglobin from 16.6% to 95%. The recovery of the new process was 58.2% in comparison to 30.3% of the conventional affinity chromatography. Furthermore, 175 mg haptoglobin production in a scaled-up process showed the method to be simple, fast, and low-cost.
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