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Title: Characterization of Plasmodium berghei glutathione synthetase. Author: Sharma SK, Banyal HS. Journal: Parasitol Int; 2011 Sep; 60(3):321-3. PubMed ID: 21377539. Abstract: Plasmodium berghei contained 0.454±0.031 U/mg of glutathione synthetyase (GS). GS was purified using solid ammonium sulfate and Sephadex G-200 from P. berghei infected mouse erythrocytes. SDS-PAGE showed purified GS as a single band protein of 70 kDa and its Km for γ-glutamylcysteine, glycine and ATP being 0.33 mM, 8.3 mM and 0.43 mM respectively with noncompetitive inhibition by GSH. The malaria parasite enzyme was optimally active at 37°C and pH 8.0-8.5. Heavy metals significantly inhibited parasite GS activity.[Abstract] [Full Text] [Related] [New Search]