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  • Title: [Abortive infection in Erwinia carotovora, as a source of nanoparticles of phage nature].
    Author: Romaniuk LV, Tovkach FI, Ivanitsa TV, Kushkina AI, Ostapchuk AN, Gorb TE.
    Journal: Mikrobiol Z; 2010; 72(6):51-7. PubMed ID: 21381318.
    Abstract:
    A possibility to obtain nanoparticles of phage nature using abortive phage infection was shown for the first time. It was found out that the nonspecific host Erwinia carotovora subsp. carotovora J2 being infected by phage ZF 40-RT80, the cells form a 100-fold surplus of capsid structures. Using the electron microscope the authors have found two types of phage capsids which differ from each other and have different modal diameters--47.5 and 71.5 nm. The found capsids pack the phage DNA which releases them under treatment of the preparations by DNAse I. A simple method of purification of capsid structures from mature virions which are formed in inconsiderable quantity in the process of abortive phage infection is proposed. The obtained results create preconditions for obtaining capsid nanoparticles as well as for studying the stages of morphogenesis and morphopoiesis of phage ZF40 without attracting special phage mutants.
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