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  • Title: Selection and characterization of a precocious line of Eimeria intestinalis, an intestinal rabbit coccidium.
    Author: Licois D, Coudert P, Boivin M, Drouet-Viard F, Provôt F.
    Journal: Parasitol Res; 1990; 76(3):192-8. PubMed ID: 2138327.
    Abstract:
    A precocious line of Eimeria intestinalis was obtained by selection for early development of oocysts in rabbits and after six consecutive passages in animals. This line (EiP) was derived from a wild strain (EiO) isolated in 1975 from the caecal content of a rabbit with coccidiosis. The prepatent period of the EiP strain was reduced from 215 h to less than 144 h, the result being that the oocyst sporulation time was the same for both lines. The excreted and unsporulated oocysts had exactly the same shape, but microscopical examination of the sporulated oocysts showed a marked difference between EiP and EiO strains. A huge refractile globule was located in each of two sporocysts of the precocious line, whereas no refractile globule was seen in the other two. The EiP line had a reproductive potential much lower (1000 times) than that of its parent strain EiO and, as judged by the weight gain, mortality and lesions that also occurred in the jejunum and above all in the ileum, its pathogenicity was substantially reduced.
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