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  • Title: Application of high-speed counter-current chromatography for preparative separation of cyclic peptides from Vaccaria segetalis.
    Author: Wang X, Dong H, Liu Y, Yang B, Wang X, Huang L.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2011 Apr 01; 879(11-12):811-4. PubMed ID: 21396894.
    Abstract:
    Following an initial clean-up step on silica gel, high-speed counter-current chromatography (HSCCC) was used to separate cyclic peptides from an extract of the seeds of Vaccaria segetalis. The two-phase solvent system used for HSCCC separation was composed of petroleum ether-ethyl acetate-methanol-water at an optimized volume ratio of 0.5:3.5:1:5. From 190 mg of crude extract, 38.0 mg of segetalin B and 28.5 mg of segetalin A were obtained with purities of 98.1% and 95.6% as determined by HPLC, respectively. The chemical structures of the target compounds were confirmed by high resolution electrospray ionization time of flight MS (HRESI-TOF-MS) and (1)H NMR analyses.
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