These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage.
    Author: Lu W, Sun Z, Tang Y, Chen J, Tang F, Zhang J, Liu JN.
    Journal: J Chromatogr A; 2011 May 06; 1218(18):2553-60. PubMed ID: 21397239.
    Abstract:
    Purification tags are robust tools that can be used to purify a variety of target proteins. However, tag removal remains an expensive and significant issue that must be resolved. Based on the affinity and the trans-splicing activity between the two domains of Ssp DnaB split-intein, a novel approach for tag affinity purification of recombinant proteins with controllable tag removal by inducible auto-cleavage has been developed. This system provides a new affinity method and avoids premature splicing of the intein fused proteins expressed in host cells. The affinity matrix can be reused. In addition, this method is compatible with his-tag affinity purification technique. Our methods provide the insights for establishing a novel recombinant protein preparation system.
    [Abstract] [Full Text] [Related] [New Search]