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  • Title: Development of a real-time PCR assay to control the illegal trade of meat from protected capercaillie species (Tetrao urogallus).
    Author: Rojas M, González I, Pavón MÁ, Pegels N, Hernández PE, García T, Martín R.
    Journal: Forensic Sci Int; 2011 Jul 15; 210(1-3):133-8. PubMed ID: 21414736.
    Abstract:
    A rapid and highly species-specific real-time polymerase chain reaction (PCR) assay has been developed for the detection of capercaillie DNA (Tetrao urogallus) in meat and meat mixtures. The method combines the use of capercaillie-specific primers, that amplify a 142bp fragment of the mitochondrial 12S rRNA gene, and a positive control primer pair that amplifies a 141bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. SYBR(®) Green dye or TaqMan(®) fluorogenic probes were used to monitor the amplification of the target genes. Results obtained with the use of TaqMan(®) probes as detection platform increased the specificity of the real-time PCR assay in comparison with the results obtained using SYBR(®) Green. The proposed real-time PCR assay represents a rapid and straightforward method for the accurate identification of capercaillie that could be used by law enforcement agencies as a tool for the control of poaching and illegal trade of meat from this protected species.
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