These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Characterization of a tryptophan 6-halogenase from Streptomyces toxytricini.
    Author: Zeng J, Zhan J.
    Journal: Biotechnol Lett; 2011 Aug; 33(8):1607-13. PubMed ID: 21424165.
    Abstract:
    Tryptophan (Trp) halogenases are found in various bacteria and play an important role in natural product biosynthesis. Analysis of the genome of Streptomyces toxytricini NRRL 15443 revealed an ORF, stth, encoding a putative Trp halogenase within a non-ribosomal peptide synthetase gene cluster. This gene was cloned into pET28a and functionally overexpressed in Escherichia coli. The enzyme halogenated both L - and D -Trp to yield the corresponding 6-chlorinated derivatives. The optimum activity was at 40°C and pH 6 giving k (cat) /K (M) value of STTH of 72,000 min(-1) M(-1). The enzyme also used bromide to yield 6-bromo-Trp.
    [Abstract] [Full Text] [Related] [New Search]