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  • Title: [Influence of aligned electrospinning poly (propylene carbonate) on axonal growth of dorsal root ganglion in vitro].
    Author: Zhao Z, Wang Y, Peng J, Qi H, Zhao B, Zhang L, Huang J, Xu W, Lu S.
    Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi; 2011 Feb; 25(2):171-5. PubMed ID: 21427845.
    Abstract:
    OBJECTIVE: Poly (propylene carbonate) (PPC), a newly reported polymer, has good biodegradability and biocompatibility. To explore the feasibility of using electrospinning PPC materials in nerve tissue engineering, and to observe the effect of aligned and random PPC materials on axonal growth of rat dorsal root ganglions (DRGs) in vitro. METHODS: Either aligned or randomly oriented sub-micron scale polymeric fiber was prepared with an electrospinning process. DRGs were harvested from 3 newborn Sprague-Dawley rats (female or male, weighing 4-6 g), and were incubated into 12-pore plate containing either aligned (the experimental group, n=6) or randomly oriented sub-micron scale polymeric fiber (the control group, n=6). The DRGs growth was observed with an inverted microscope; at 7 days immunofluorescent staining and scanning electronic microscope (SEM) observation were performed to quantify the extent of neurite growth and Schwann cells (SCs) migration. RESULTS: Either aligned or random fibers were fabricated by an electrospinning process. The diameter of the individual fiber ranged between 800 nm and 1200 nm. In aligned PPC material, 90% fibers arranged in long axis direction, but the fibers in random PPC material arranged in all directions. The DRGs grew well in 2 PPC materials. On the aligned fiber film, the majority of neurite growth and SCs migration from the DRGs extended unidirectionally, parallel to the aligned fibers; however, neurite growth and SCs migration on the random fiber films oriented randomly. The extents of neurite growth were (2 684.7 +/- 994.8) microm on the aligned fiber film and (504.7 +/- 52.8) microm on the random fiber films, showing significant difference (t = -5.360, P = 0.000). The distances of SCs migration were (2 770.6 +/- 978.4) microm on the aligned fiber film and (610.2 +/- 56.3) microm on the random fiber films, showing significant difference (t = -5.400, P = 0.000). The extent of neurite growth was fewer than the distances of SCs migration in 2 groups. CONCLUSION: The orientation structure of sub-micron scale fibers determines the orientation and extent of DRGs neurite growth and SCs migration. Aligned electrospinning PPC fiber is proved to be a promising biomaterial for nerve regeneration.
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