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  • Title: Purification of 6×His-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles.
    Author: Chen Y, Jiang P, Liu S, Zhao H, Cui Y, Qin S.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2011 Apr 15; 879(13-14):993-7. PubMed ID: 21435956.
    Abstract:
    Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6×His-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6×His-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25°C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6×His-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6×His-tagged phycobiliprotein.
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