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  • Title: [Optimization of ultrahigh pressure extraction for polydatin and resveratrol from Polygonum cuspidatum by using uniform design].
    Author: Liao G, He S, Zhang Z.
    Journal: Zhongguo Zhong Yao Za Zhi; 2010 Dec; 35(24):3282-6. PubMed ID: 21438390.
    Abstract:
    OBJECTIVE: To optimize the ultrahigh pressure extraction (UPE) process of polydatin and resveratrol from Polygonum cuspidatum by using uniform design. METHOD: On the basis of single factor screening, the uniform design was adopted for getting the optimal technique parameters. The optimum result of UPE was compared with conventional extractions. RESULT: The optimal conditions of UPE for polydatin and resveratrol were that the solvent was 55% ethanol, the ratio of solvent to material( mL: g) was 30, the extraction pressure was 170 MPa, and the extraction time was 120 second. With this extracting process, the extraction yield of polydatin and resveratrol were 14.29 and 2.53 mg x g(-1), respectively. The extraction yield of polydatin was 46.1% higher than the heat reflux extraction, 6.4% higher than the ultrasonic extraction and 28.5% higher than the microwave extraction, while the yield of resveratrol was 67.5% higher than the heat reflux extraction, 29.7% higher than the ultrasonic extraction and 24.6% higher than the microwave extraction, respectively. CONCLUSION: As a novel extraction technology for Chinese herbal medicine, the UPE procedure has higher extraction yield, lower extracting temperature, shorter extacting time and less power consumption. The UPE has provided a brand-new method for extraction of polydatin and resveratrol from P. cuspidatum.
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