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  • Title: Isolation and identification of epithelial-like cells in culture by a collagenase-separation technique.
    Author: Kanoza RJ, Brunette DM, Purdon AD, Sodek J.
    Journal: In Vitro; 1978 Sep; 14(9):746-53. PubMed ID: 214390.
    Abstract:
    An operational criterion for the identification and isolation of epithelial-like (E) cells, based on their ability to cover and protect a collagen gel from the action of collagenase, has been developed. The E cells isolated by this collagenase-separation technique (CST) exhibited the ultrastructural features, including desmosomes and abundant tonofilaments, that are considered characteristic of this cell type. Unlike confluent cultures of fibroblast-like (F) cells, E cells were not found to have large external transformation-sensitive (LETS) protein on their surface membranes. The CST provides a nondestructive and efficient means of identifying and isolating E cells from mixed populations.
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