These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Anti-CD3 antibody-induced activated killer cells subsets of killer cells that mediate fast or slow lytic reactions.
    Author: Ting CC, Hargrove ME, Henrich P.
    Journal: Immunol Invest; 1990 Aug; 19(4):347-61. PubMed ID: 2145218.
    Abstract:
    The present study has characterized two sets of alpha CD3-induced activated killer cells (CD3-AK). A 2 to 4-h 51Cr release assay or triton-treated 125IUdR release assay demonstrated that CD3-AK cultured in the continuous presence of alpha CD3 (CD3-AK+) mediated fast lysis. A 20-h 51Cr or 125I-deoxyuridine release assay demonstrated that CD3-AK cultured in the absence of alpha CD3 (CD3-AK-) mediated slow lysis. Activating the TCR-CD3 complex of CD3-AK- cells with alpha CD3 for 2 h enabled the killer cells to mediate fast lysis. The activation process was inhibited by H-7, a protein kinase C (PKC) inhibitor. Conversely, removal of alpha CD3 from CD3-AK+ cell cultures for 24 h resulted in almost complete loss of the ability of CD3-AK+ cells to mediate fast lysis, but they still retained the ability to mediate slow lysis. It appeared that constant perturbation of CD3 by alpha CD3 maintained the CD3-AK+ cells in an active state, and thus, they were able to mediate fast lysis. On the other hand, activation by a 2-h incubation with PMA could convert the noncytolytic CD3-AK- cells to be cytolytic in slow lysis and to augment the slow lytic reactions mediated by CD3-AK- cell with low cytolytic activity. These results were confirmed by triton-treated 125IUdR release assay which could detect early DNA-release. Thus it appeared that activation of CD3-AK cells with T cell activation signals that bypassed TCR (such as PMA) could induce slow lysis. In the effector phase of lytic reactions, the fast lytic reaction was relatively resistant to inhibition by H-7, whereas the slow lytic reaction was susceptible to H-7 inhibition, indicating that fast lysis was PKC independent and slow lysis was PKC dependent. It was further found that H-7 inhibition was at an early stage of slow lysis, suggesting that a PKC dependent activation process preceded the PKC independent lytic process. These findings indicated that the CD3-AK- cells were in a less activated state which required further activation to turn on their lytic machinery to initiate the lytic reaction.
    [Abstract] [Full Text] [Related] [New Search]