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  • Title: Translational efficiency of phi X174 lysis gene E is unaffected by upstream translation of the overlapping gene D reading frame.
    Author: Bläsi U, Nam K, Lubitz W, Young R.
    Journal: J Bacteriol; 1990 Oct; 172(10):5617-23. PubMed ID: 2145264.
    Abstract:
    The lysis gene E of bacteriophage phi X174 is entirely embedded in gene D. Expression studies of genes D and E in Escherichia coli minicells and lysis times obtained in the presence or absence of D translation showed that the simultaneous expression of gene D does not affect protein E production. Thus, unlike other overlapping gene pairs, gene E expression is independent from the upstream translation of gene D. lacZ fusion studies and primer extension inhibition analysis (toeprinting) revealed an intrinsically weak E ribosome-binding site, which seems to be the major factor determining the low expression rate of the gene and thus proper scheduling of cell lysis.
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