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  • Title: Repeated fed-batch fermentation using biosensor online control for citric acid production by Yarrowia lipolytica.
    Author: Moeller L, Grünberg M, Zehnsdorf A, Aurich A, Bley T, Strehlitz B.
    Journal: J Biotechnol; 2011 May 20; 153(3-4):133-7. PubMed ID: 21458506.
    Abstract:
    Biosensor-controlled substrate feeding was used in a citric acid production process with the yeast strain Yarrowia lipolytica H222 with glucose as the carbon source. The application of an online glucose biosensor measurement facilitated the performance of long-time repeated fed-batch process with automated bioprocess control. Ten cycles of repeated fed-batch fermentation were carried out in order to validate both the stability of the microorganism for citric acid production and the robustness of the glucose biosensor in a long-time experiment. In the course of this fermentation with a duration of 553 h, a slight loss of productivity from 1.4 g/(L×h) to 1.1 g/(L×h) and of selectivity for citric acid from 91% to 88% was observed. The glucose biosensor provided 6,227 measurements without any loss of activity.
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