These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: RNA interference of WdFKS1 mRNA expression causes slowed growth, incomplete septation and loss of cell wall integrity in yeast cells of the polymorphic, pathogenic fungus Wangiella (Exophiala) dermatitidis.
    Author: Guo P, Szaniszlo PJ.
    Journal: Med Mycol; 2011 Nov; 49(8):806-18. PubMed ID: 21469912.
    Abstract:
    As one of the main components of the fungal cell wall, β-1,3-glucan provides the mechanical strength to protect fungal protoplasts. The enzyme responsible for the synthesis of β-1,3-glucans in fungi is β-1,3-glucan synthase. Here we report the cloning, sequencing and characterization of the WdFKS1 gene, which in the pathogenic fungus Wangiella dermatitidis encodes the catalytic domain of its β-1, 3-glucan synthase. Because our research suggested that WdFKS1 is a single copy essential gene, we used RNA interference to reduce its expression. Reduction of the WdFKS1 messenger retarded growth and caused the loss of cell wall integrity of yeast cells, but not hyphae or sclerotic cells. We suggest that the WdFKS1 in this polymorphic agent of phaeohyphomycosis is not only required for cell wall construction and maintenance, but also is involved in septum formation.
    [Abstract] [Full Text] [Related] [New Search]