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  • Title: [A study of gene therapy for beta-thalassemia. I. Retroviral-mediated transfer of human beta-globin gene into Friend cells].
    Author: Shu H.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 1990 Aug; 12(4):252-6. PubMed ID: 2147584.
    Abstract:
    We used a replication-defective retrovirus vector containing the human beta-globin gene to transfect the ecotropic packaging cell line psi-2, and then harvested the virus supernatant to infect the amphotropic packaging cell line PA317. As a result, a recombinant vector producing cell line (PIW beta) with a titer of 1.1 x 10(5) CFU/ml was isolated. By infecting Friend cells with this high titer virus vector, high efficiency gene transfer was achieved. Southern blot hybridization analysis indicated that the intact human beta-globin gene was stably integrated into the genome of the target cells. Our results provide a useful reference for further studies of gene therapy of beta-thalassemia.
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