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  • Title: Conjugation of siRNA with comb-type PEG enhances serum stability and gene silencing efficiency.
    Author: Gunasekaran K, Nguyen TH, Maynard HD, Davis TP, Bulmus V.
    Journal: Macromol Rapid Commun; 2011 Apr 19; 32(8):654-9. PubMed ID: 21480423.
    Abstract:
    A thiol-modified siRNA targeting the enhanced green fluorescence protein (eGFP) gene was conjugated with RAFT-synthesized, pyridyl disulfide-functional poly(PEG methyl ether acrylate)s (p(PEGA)s). siRNA-p(PEGA) conjugates demonstrated significantly enhanced in vitro serum stability and nuclease resistance compared to the unmodified and thiol-modified siRNA. The complexes of siRNA-p(PEGA) conjugates with a fusogenic peptide, KALA ((+)/(-) = 2) inhibited the protein expression approximately 28-fold more than the KALA complex of the unmodified siRNA. The protein inhibition caused by siRNA-p(PEGA)-KALA complexes (56 ± 5%-58 ± 3% of the fluorescence expressed in non-treated cells) was comparable to the effect of the unmodified siRNA-lipofectamine complex (77 ± 7%).
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