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  • Title: A xylanase gene directly cloned from the genomic DNA of alkaline wastewater sludge showing application potential in the paper industry.
    Author: Zhao Y, Luo H, Meng K, Shi P, Wang G, Yang P, Yuan T, Yao B.
    Journal: Appl Biochem Biotechnol; 2011 Sep; 165(1):35-46. PubMed ID: 21484273.
    Abstract:
    A xylanase gene, aws-2x, was directly cloned from the genomic DNA of the alkaline wastewater sludge using degenerated PCR and modified TAIL-PCR. The deduced amino acid sequence of AWS-2x shared the highest identity (60%) with the xylanase from Chryseobacterium gleum belonging to the glycosyl hydrolase GH family 10. Recombinant AWS-2x was expressed in Escherichia coli BL21 (DE3) and purified to electrophoretic homogeneity. The enzyme showed maximal activity at pH 7.5 and 55 °C, maintained more than 50% of maximal activity when assayed at pH 9.0, and was stable over a wide pH range from 4.0 to 11.0. The specific activity of AWS-2x towards hardwood xylan (beechwood and birchwood xylan) was significantly higher than that to cereal xylan (oat spelt xylan and wheat arabinoxylan). These properties make AWS-2x a potential candidate for application in the pulp and paper industry.
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