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  • Title: [Electron microscopic study of glycocalyx formation in different functional states of the frog bladder epithelial cells].
    Author: Komissarchik IaIu, Snigirevskaia ES, Shakhmatova EI, Natochin IuV.
    Journal: Tsitologiia; 1978 Aug; 20(8):891-5. PubMed ID: 214913.
    Abstract:
    The apical part of the urinary bladder granular cells contains oval granules filled with a homogenous substance; close to the plasma membrane granules with tubular element are disposed. A preliminary 30 min fixation of the bladder epithelium with 0.1--0.5% glutaraldehyde followed by 2.5% glutaraldehyde post-fixation rather increased the number of granules with the orderly distributed inner material. The membrane of these granules includes in to the plasma membrane. The loosening of the inner material takes place and the fibrillar elements similar in structure to the glycocalix fibrilles attach to the external surface of the cell plasma membrane.
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