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  • Title: [Effects of verapamil on the proliferation, DNA synthesis and migration of human hepatoma cell line HepG2].
    Author: Liang NL, Wei Y, Wu WC, Liu XJ, Yang L.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2011 Mar; 42(2):227-30. PubMed ID: 21500559.
    Abstract:
    OBJECTIVE: To study the effects of verapamil (VR) on proliferation, DNA synthesis and migration of human hepatoma cell line HepG2. METHODS: HepG2 cells were treated with different concentration of VR (0,1, 5, 10, 15, 20 microg/mL) for different time point. Cell proliferation was evaluated by MTT assay and DNA synthesis was determined by 5-ethynyl-2'-deoxyuridine (Edu) fluorescent assay. The migration of cells was measured by Scratch wound migration assay. RESULTS: MTT assay demonstrated that the different concentration of VR (1, 5, 10, 15, 20 microg/mL) could inhibit the proliferation of HepG2 cells after 24 h and 48 h treatment (P < 0.05) except 1, 5 microg/mL group processing for 24 h (P > 0.05). Edu fluorescent assay showed that, the proportion of HepG2 cells in the stage of DNA synthesis was (51.5 +/- 3.78)%, (52.4 +/- 3.26)%, (53.1 +/- 1.94)%, (39.6 +/- 4.25)%, (40.2 +/- 2.67)%, (42.6 +/- 3.13)% respectively for the treatment of 0, 1, 5, 10, 15, 20 microg/mL concentration of VR for 24 h. DNA synthesis in the cells treated with VR (10, 15 and 20 microg/mL concentration) was lower than that in the cells treated with 0, 1, 5 microg/mL concentrations of VR (P < 0.05). Scratch wound migration assay indicated that treatment with VR (10, 15 and 20 microg/mL) for 24 h significantly inhibited cells migration distance (P < 0.05). CONCLUSION: Calcium channel blocker VR could inhibit the cell proliferation and migration of human hepatoma cell line HepG2.
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