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  • Title: [A method for differential identification of group C and G streptococci with PCR].
    Author: Il'iasov IuIu, Biswas I, Totolian AA, Dmitriev AV.
    Journal: Klin Lab Diagn; 2011 Feb; (2):40-3. PubMed ID: 21506385.
    Abstract:
    The paper describes a rapid method for PCR identification of Groups C and G streptococci (Streptococcus dysgalactiae subspecies equisimilis, Streptococcus constellatus, and Streptococcus anginosus) that cause human disease. Species-specific regions of the cpn60 gene encoding heat shock protein GroEL (HSP60) were chosen as markers for PCR diagnosis; three pairs of primers were constructed for these regions, each of which was peculiar to the specific type. The method was tested on a large collection of pathogenic streptococci of different serogroups isolated from man and animals; its specificity was shown to identify S. dysgalactiae subsp. equisimilis, S. constellatus, and S. anginosus. The proposed method has all benefits of PCR-based techniques, which enables it to be used for the purposes of molecular epidemiology.
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