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  • Title: Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates.
    Author: Deccache Y, Irenge LM, Savov E, Ariciuc M, Macovei A, Trifonova A, Gergova I, Ambroise J, Vanhoof R, Gala JL.
    Journal: J Microbiol Methods; 2011 Jul; 86(1):115-8. PubMed ID: 21514328.
    Abstract:
    Rapid and reliable assessment of Acinetobacter baumannii resistance to quinolones was successfully achieved through pyrosequencing of the gyrA and parC quinolone-resistance determining regions. A strong correlation was found between quinolone resistance and mutations in gyrA codon 83 and/or in the parC gene (codons 80 or 84). Absence of QRDR mutations was associated with susceptibility to quinolones.
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