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  • Title: Re-assessment of phenotypic identifications of Bacteroides putredinis to Alistipes species using molecular methods.
    Author: Tyrrell KL, Warren YA, Citron DM, Goldstein EJ.
    Journal: Anaerobe; 2011 Jun; 17(3):130-4. PubMed ID: 21527349.
    Abstract:
    Alistipes (previously Bacteroides) are strictly anaerobic gram-negative rods that resemble the Bacteroides fragilis group in that most species are bile-resistant and indole-positive; however, they are only weakly saccharolytic and most species produce light brown pigment only on laked rabbit blood agar. In this retrospective study, we re-identified 18 organisms previously identified phenotypically as "Bacteroides putredinis-like", but that did not produce pigment on routine media. The strains were identified with 16S rDNA sequencing and pigment production was evaluated on several different culture media. Only 12/18 strains had molecular identifications of Alistipes species, while the remaining strains phylogenetically resembled Butyricimonas and Odoribacter spp. Pigment production was not a reliable test for those Alistipes strains that are described as pigment producers.
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