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  • Title: A radiochemical method for the determination of transketolase activity in erythrocyte hemolysates.
    Author: Reijnierse GL, van der Horst AR, de Kloet K, Voorhorst CD.
    Journal: Clin Chim Acta; 1978 Dec 15; 90(3):259-68. PubMed ID: 215350.
    Abstract:
    A radiochemical method for the determination of transketolase activity is reported. It is based on incubation of erythrocyte hemolysates with radioactive ribose 5-phosphate followed by isolation of sedoheptulose 7-phosphate on anion-exchange columns. The experimental conditions are discussed and the presence of phosphatase activity in the incubation mixture is demonstrated. The proposed method is compared with a current colorimetric method. Reference values are given for the transketolase activity and the thiamine pyrophosphate effect.
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