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  • Title: Does 3,5,dibromo-4-nitrosobenzene sulphonate spin trap superoxide radicals?
    Author: Nazhat NB, Yang G, Allen RE, Blake DR, Jones P.
    Journal: Biochem Biophys Res Commun; 1990 Jan 30; 166(2):807-12. PubMed ID: 2154221.
    Abstract:
    Pulse radiolysis studies show that the spin trap 3,5,dibromo-4-nitrosobenzene sulphonate (I) reacts rapidly with O2.- but the product formed is very unstable. No radicals were detected in ESR studies of solutions of I after reaction with O2.- formed by gamma-radiolysis. Evidence is presented that the stable radical observed by some, but not all workers, following exposure of I to the O2.(-)-generating xanthine/xanthine oxidase system, is produced by a peroxidatic oxidation using hydrogen peroxide formed by O2-. dismutation and that formation of this radical depends on the presence of peroxidase activity in the xanthine oxidase sample employed.
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