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  • Title: Secretion of biologically-active human interferon-β by Bacillus subtilis.
    Author: Kakeshita H, Kageyama Y, Endo K, Tohata M, Ara K, Ozaki K, Nakamura K.
    Journal: Biotechnol Lett; 2011 Sep; 33(9):1847-52. PubMed ID: 21544609.
    Abstract:
    Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1.5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.
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