These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Mapping of low abundance latency-associated RNA in the trigeminal ganglia of mice latently infected with herpes simplex virus type 1.
    Author: Mitchell WJ, Lirette RP, Fraser NW.
    Journal: J Gen Virol; 1990 Jan; 71 ( Pt 1)():125-32. PubMed ID: 2154530.
    Abstract:
    During herpes simplex virus type 1 (HSV-1) latent infection of the mouse trigeminal ganglion there is limited viral gene expression. The latency-associated transcripts (LAT) map approximately to the PstI-MluI fragment within the BamHI B and BamHI E fragments (long repeat regions) of the viral genome. Additional weak hybridization signals have been detected by in situ hybridization that correspond to transcription from HSV-1 DNA fragments adjacent tot he PstI-MluI fragment. We mapped the region encoding this additional transcription. This minor latency-associated RNA (m-LAT) was shown to map to a group of contiguous fragments (approximately 8.3 kb of DNA), which are adjacent to the 3' end of LAT and to a (2.0 kb) fragment adjacent to the 5' end of the LAT. Using single-stranded probes in in situ hybridization experiments, we showed that the KpnI-BamHI and BamHI-SacI regions of m-LAT are transcribed in a rightward direction within the long internal repeat region. This low abundance RNA may be related to the previously described LAT.
    [Abstract] [Full Text] [Related] [New Search]