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  • Title: Identification of a new membrane-associated polypeptide specified by the coronavirus infectious bronchitis virus.
    Author: Smith AR, Boursnell ME, Binns MM, Brown TD, Inglis SC.
    Journal: J Gen Virol; 1990 Jan; 71 ( Pt 1)():3-11. PubMed ID: 2154538.
    Abstract:
    Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.
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