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  • Title: Development of an immunochromatographic strip test for rapid detection of melamine in raw milk, milk products and animal feed.
    Author: Li X, Luo P, Tang S, Beier RC, Wu X, Yang L, Li Y, Xiao X.
    Journal: J Agric Food Chem; 2011 Jun 08; 59(11):6064-70. PubMed ID: 21548621.
    Abstract:
    A simple, rapid and sensitive immunogold chromatographic strip test based on a monoclonal antibody was developed for the detection of melamine (MEL) residues in raw milk, milk products and animal feed. The limit of detection was estimated to be 0.05 μg/mL in raw milk, since the detection test line on the strip test completely disappeared at this concentration. The limit of detection was 2 μg/mL (or 2 μg/g) for milk drinks, yogurt, condensed milk, cheese, and animal feed and 1 μg/g for milk powder. Sample pretreatment was simple and rapid, and the results can be obtained within 3-10 min. A parallel analysis of MEL in 52 blind raw milk samples conducted by gas chromatography-mass spectrometry showed comparable results to those obtained from the strip test. The results demonstrate that the developed method is suitable for the onsite determination of MEL residues in a large number of samples.
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