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  • Title: Identification of alpha 2- and alpha 3-subunits of the GABAA-benzodiazepine receptor complex purified from the brains of young rats.
    Author: Fuchs K, Adamiker D, Sieghart W.
    Journal: FEBS Lett; 1990 Feb 12; 261(1):52-4. PubMed ID: 2155135.
    Abstract:
    Polyclonal antibodies were raised to synthetic amino acid sequences of the bovine GABAA receptor alpha 2- and alpha 3-subunits and purified by affinity chromatography on a column coupled with the respective peptide. Anti-peptide alpha 2(416-424) and anti-peptide alpha 3(459-467) antibodies immunoprecipitated GABAA receptors and recognized a protein of 53 kDa (P53) and 59 kDa (P59), respectively, in Western blots of GABAA receptors purified from the brains of 5-10 day old rats. P53 as well as P59 are specifically photolabeled by [3H]flunitrazepam and are recognized by the alpha-subunit specific monoclonal antibody bd 28.
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