These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Enhanced penetration of zona-free hamster ova by sperm prepared by Nycodenz and Percoll gradient centrifugation.
    Author: Serafini P, Blank W, Tran C, Mansourian M, Tan T, Batzofin J.
    Journal: Fertil Steril; 1990 Mar; 53(3):551-5. PubMed ID: 2155143.
    Abstract:
    The effect of sperm penetration capacity after selection procedures using Percoll (Pharmacia AB, Uppsala, Sweden) and Nycodenz (Nycomed Diagnostics, Oslo, Norway) gradient centrifugation was compared with double-washed and swim-up in 47 subfertile men. The results of sperm motility, velocity, and amplitude lateral head displacement showed no significant improvement with the centrifugation procedures. The sperm penetration assay results obtained with double-washed and swim-up technique were poor (2.7% +/- 1.7%), however, a significant enhancement was obtained by Percoll (16.3% +/- 3.7%) and Nycodenz (15.8% +/- 3.3%) processing. Nycodenz centrifugation allowed sperm penetration of zona-free hamster ova at comparable rates to Percoll separation.
    [Abstract] [Full Text] [Related] [New Search]