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  • Title: Apparent absence of Na+/H+ antiport activity in the two-cell mouse embryo.
    Author: Baltz JM, Biggers JD, Lechene C.
    Journal: Dev Biol; 1990 Apr; 138(2):421-9. PubMed ID: 2156738.
    Abstract:
    We have used the pH-sensitive dye BCECF to investigate the regulation of intracellular pH (pHi) by two-cell stage mouse embryos in bicarbonate-free medium. There is no indication of a Na+/H+ antiport active in regulating pHi, as recovery from acid-loading was insensitive to amiloride, ethylisopropylamiloride, or the absence of extracellular Na+. Instead, protons appear to be in equilibrium across the plasma membrane, as indicated by the response of pHi to changes in external K+. The embryos have an intracellular buffering power in the normal range (25.3 mM/pH); their apparent permeability to protons is, however, very high (0.22 cm/sec).
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