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  • Title: Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique.
    Author: Titheradge MA, Coore HG.
    Journal: Biochem J; 1975 Sep; 150(3):553-6. PubMed ID: 2157.
    Abstract:
    An "inhibitor-stop" technique has been developed for measuring initial rates of pyruvate transport into mitochondria. The technique uses alpha-cyano-3-hydroxycinnamate as the inhibitor and separates the mitochondria from the radioactive medium by Millipore filtration. Observed rates depend on availability of hydroxyl and other exchangeable anions within the mitochondrial matrix.
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