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  • Title: A novel biosensor based on glucose oxidase for activity determination of α - amylase.
    Author: Altug C, Mengulluoglu U, Kurt E, Kaya S, Dinckaya E.
    Journal: Artif Cells Blood Substit Immobil Biotechnol; 2011 Oct; 39(5):298-303. PubMed ID: 21574907.
    Abstract:
    A glucose oxidase-based biosensor was developed for the determination of α-amylase activity. The determination method is based on monitoring the decrease in dissolved oxygen concentration related to the starch concentration, for which starch gives a reaction with α-amylase. Optimization parameters, including glucose oxidase amount, gelatin amount, and glutaraldehyde percentage for cross-linking, were investigated. The effects of pH, buffer system, and temperature on the biosensor system were also investigated. The biosensor had a linear relation to α-amylase activity and good measurement correlation between 0.66 and 9.83 U/ml. In sample analysis studies, α-amylase activity in baker's yeast was determined by the biosensor.
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