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  • Title: [Optimization of nutrient medium for cultivation of Bacillus subtilis IMV V-7023].
    Author: Tsarenko IIu, Roĭ AA, Kudrish IK.
    Journal: Mikrobiol Z; 2011; 73(2):13-9. PubMed ID: 21598654.
    Abstract:
    Nutrient medium for cultivation of Bacillus subtilis IMV V-7023 was optimized by the method of the orthogonal Latin rectangles. Optimum concentrations were investigated in the medium of carbon source (15.0 g/l of molasses), nitrogen (2.0 g/l of corn extract) and content of phosphorus-containing inorganic salts (0.4 g/l). When growing this strain in periodic conditions at 28 degrees C, the value of oxygen mass transport in the liquid (0.4-0.6 g O2/l per 1 h) and the initial number of cells of each bacterial species was 1 x 10(6) cells/ml. The numbers of bacteria reached the maximum after 24 hours of cultivation and corresponded to 1.3 x 10(10) cells/ml. Nutrient medium that was optimized can be recommended for cultivation of B.subtilis IMV V-7023 in production conditions. The paper is presented in Russian.
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